Related Experiment Video
Updated: Apr 27, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
The effect of substrate concentration fluctuation on the performance of high-rate denitrifying reactor
Wei Li1, Ping Zheng1, Jiqiang Zhang2
1Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
Abstract:
A high-rate denitrifying process is sensitive to the operation conditions and the substrate concentration fluctuation can lead to the deterioration or even collapse of process performance. The results of this study showed that the effect of substrate concentration fluctuation on the high-rate denitrification process was related to the substrate concentration and shock duration. The effect of substrate concentration was greater than that of shock duration and nitrate conversion was more sensitive than methanol conversion. The response of denitrification performance was related to the loading saturation (maximum loading rate/loading capacity ratio). When the loading saturation was lower than 32%, the high-rate denitrification process could stay in pseudo steady state, otherwise it would easily lose stability. The response of denitrification performance could be divided into three periods. The performance deterioration of high-rate denitrifying process could be attributed to the overload trigger and the toxicity of free nitrous acid.
More Related Videos
Related Concept Videos
Enzyme Kinetics
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Introduction to Enzyme Kinetics
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Bioreactor Controls-II
Bioreactor Design and Operational System
Bioreactor Controls-I
Concentration and Rate Law
For example, in a generic reaction aA + bB ⟶ products, where a and b are stoichiometric coefficients, the rate law can be written as:

