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Related Experiment Video

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Surface plasmon resonance-based infrared biosensor for cell studies with simultaneous control.

Alexander Zilbershtein1, Amir Bein2, Vladislav Lirtsman1

  • 1Hebrew University of Jerusalem, The Racah Institute of Physics, 91904 Jerusalem, Israel.

Journal of Biomedical Optics
|June 28, 2014
PubMed
Summary
This summary is machine-generated.

We developed a novel double-chamber biosensor for label-free cell monitoring. This infrared surface plasmon sensor offers higher sensitivity for tracking cell responses to stimuli.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biosensing Technology

Background:

  • Continuous monitoring of cell morphology is crucial for understanding cellular responses to stimuli.
  • Existing biosensing methods often lack the sensitivity or real-time capabilities required for dynamic cellular studies.
  • Label-free techniques are desirable to avoid interference with cellular processes.

Purpose of the Study:

  • To develop and validate a novel label-free infrared surface plasmon biosensor for real-time monitoring of cell morphological changes.
  • To assess the performance of a double-chamber flow cell design for comparative cellular studies.
  • To demonstrate the biosensor's capability in tracking cellular responses to specific stimuli, such as changes in extracellular calcium concentration.

Main Methods:

  • Utilized an infrared surface plasmon resonance (SPR) biosensor.
  • Incorporated a double-chamber flow cell to divide a cell monolayer into treated and control sections.
  • Performed experiments using IEC-18 cell cultures exposed to varying extracellular Ca2+ concentrations.
  • Monitored real-time morphological changes in the cell layers.

Main Results:

  • The double-chamber flow cell enabled simultaneous treatment and control of cell cultures.
  • The biosensor successfully tracked the kinetic response of IEC-18 cells to extracellular Ca2+ variations.
  • The developed method demonstrated an order of magnitude higher sensitivity compared to single-chamber biosensor configurations.
  • Label-free, continuous monitoring of cellular morphological changes was achieved.

Conclusions:

  • The double-chamber infrared surface plasmon biosensor is a sensitive and effective tool for label-free, continuous monitoring of cell culture responses.
  • This innovative setup allows for precise investigation of cellular dynamics under various experimental conditions.
  • The enhanced sensitivity offers significant advantages for studying subtle cellular morphological changes.