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Updated: Apr 27, 2026

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
Published on: October 31, 2014
MoxT toxin of Bacillus anthracis exhibits sequence specific ribonuclease activity
Shashikala Verma1, Rakesh Bhatnagar1
1Laboratory of Genetic Engineering and Molecular Biology, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
Abstract:
MoxXT module of Bacillus anthracis encodes MoxX, a labile protein and MoxT, a ribonuclease. However, mechanism of cleavage of RNA by MoxT has not been explored till date. In the present study, we have demonstrated that MoxT is a sequence specific ribonuclease which recognizes UACAU sequence in ss RNA and cleaves between U and A. Moreover, cleavage of RNA requires 2' OH group of first residue, i.e. U of UACAU RNA sequence. An interesting finding which makes it distinct from the other MazF family toxins was also observed, i.e. its ability to cleave RNA in DNA-RNA hybrid.
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