Supercooling enables long-term transplantation survival following 4 days of liver preservation
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Updated: Apr 27, 2026

Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels
Published on: July 3, 2025
Tim A Berendsen1, Bote G Bruinsma2, Catheleyne F Puts2
11] Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. [2] Department of Surgery, University Medical Center, Utrecht, the Netherlands.
Abstract:
The realization of long-term human organ preservation will have groundbreaking effects on the current practice of transplantation. Herein we present a new technique based on subzero nonfreezing preservation and extracorporeal machine perfusion that allows transplantation of rat livers preserved for up to four days, thereby tripling the viable preservation duration.

