Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Caspases01:24

Caspases

8.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reconstitution of the Alzheimer's Disease Tau Core Structure from Recombinant Tau<sup>297-391</sup> Yields Variable Quaternary Structures as Seen by Negative Stain and Cryo-EM.

Biochemistry·2023
Same author

Aberrant vascular architecture in the hippocampus correlates with tau burden in mild cognitive impairment and Alzheimer's disease.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2023
Same author

Cases of familial idiopathic normal pressure hydrocephalus implicate genetic factors in disease pathogenesis.

Cerebral cortex (New York, N.Y. : 1991)·2023
Same author

Reply: Soluble oligomers or insoluble fibrils?

Acta neuropathologica·2023
Same author

Age-Dependent Increase in Tau Phosphorylation at Serine 396 in Huntington's Disease Prefrontal Cortex.

Journal of Huntington's disease·2023
Same author

APOE2 gene therapy reduces amyloid deposition, and improves markers of neuroinflammation and neurodegeneration in a mouse model of Alzheimer disease.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Apr 27, 2026

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

7.7K

Measuring caspase activity in vivo.

Samantha B Nicholls1, Bradley T Hyman1

  • 1MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Alzheimer's Disease Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, USA.

Methods in Enzymology
|June 30, 2014
PubMed
Summary
This summary is machine-generated.

Caspases, proteases crucial for apoptosis, are key targets for disease research. Various technologies now exist to measure caspase activity in vivo, aiding in apoptosis research and drug development.

Keywords:
ApoptosisCaspaseFRETGFPLuciferase

More Related Videos

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

8.6K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.6K

Related Experiment Videos

Last Updated: Apr 27, 2026

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

7.7K
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

8.6K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Death Research

Background:

  • Caspases are essential proteases regulating apoptosis (programmed cell death).
  • Dysregulation of apoptosis is implicated in numerous diseases, highlighting the need for accurate measurement tools.
  • Caspase activity is a critical determinant of cell fate.

Purpose of the Study:

  • To review technologies for measuring caspase activity in vivo.
  • To discuss the applications and mechanisms of various caspase activity detection reagents.
  • To provide guidance on selecting appropriate methods for specific research needs.

Main Methods:

  • Review of existing literature on caspase activity measurement technologies.
  • Categorization of reagents based on detection principles (e.g., small molecules, protein-based reporters).
  • Analysis of in vivo and cell-based applications for each technology.

Main Results:

  • A wide array of technologies for measuring caspase activity in vivo are available.
  • These technologies include small molecule PET imaging reagents and fluorescent/luminescent protein reporters.
  • Each technology has specific applications and mechanisms for detecting caspase activity.

Conclusions:

  • Understanding the available technologies is crucial for effective apoptosis research.
  • The choice of technology depends on the specific application, whether in vitro or in vivo.
  • Accurate measurement of caspase activity aids in disease understanding and therapeutic development.