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[FGF-21 protects H9c2 cardiomyoblasts against hydrogen peroxide-induced oxidative stress injury]
Abstract:
Fibroblast growth factor-21 (FGF-21) is an important metabolism regulator, however, whether FGF-21 has effects on cardiovascular remains unclear. In this study, H2O2-induced injury in H9c2 cells was used as a cell model, the anti-apoptosis potential and mechanism of FGF-21 against oxidative injury were evaluated by MTT assay, flow cytometry assay and real-time PCR. The results showed that FGF-21 could increase the cell survival of H2O2-induced injury in H9c2 cells and prevent H9c2 cells from oxidative stress-induced apoptosis. Furthermore, FGF-21 can elevate SOD activity and regulate Bcl-2/Bax expression in H9c2 cells. The results suggest that FGF-21 have protective effect against the H2O2-induced apoptosis in H9c2 cells.
Insights
Fibroblast growth factor-21 (FGF-21) protects heart cells from oxidative stress and apoptosis. This study shows FGF-21 enhances cell survival and regulates key proteins involved in cell death.
Area of Science:
- Cardiovascular Biology
- Cellular Stress Response
- Metabolic Regulation
Background:
- Fibroblast growth factor-21 (FGF-21) is a key metabolic regulator.
- The role of FGF-21 in cardiovascular protection against oxidative injury is not well understood.
Purpose of the Study:
- To investigate the protective effects of FGF-21 against oxidative stress-induced apoptosis in H9c2 cells.
- To elucidate the underlying mechanisms of FGF-21's cardioprotective action.
Main Methods:
- H9c2 cells were subjected to hydrogen peroxide (H2O2)-induced oxidative injury.
- Cell viability was assessed using MTT assay.
- Apoptosis was evaluated by flow cytometry.
- Gene expression related to apoptosis was analyzed using real-time PCR.
- Superoxide dismutase (SOD) activity was measured.
Main Results:
- FGF-21 significantly increased cell survival in H2O2-injured H9c2 cells.
- FGF-21 demonstrated anti-apoptotic effects, preventing cell death induced by oxidative stress.
- FGF-21 treatment elevated SOD activity, indicating enhanced antioxidant defense.
- FGF-21 modulated the expression of Bcl-2 and Bax proteins, key regulators of apoptosis.
Conclusions:
- FGF-21 exhibits a significant protective effect against H2O2-induced apoptosis in H9c2 cells.
- FGF-21 confers cardioprotection by enhancing antioxidant capacity and regulating apoptosis-related gene expression.
