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Monoclonal antibodies specific for variable and constant domains of murine lambda chains
1Institute for Immunology and Rheumatology, University of Oslo, Norway.
Scandinavian Journal of Immunology
|March 1, 1989
Summary
Researchers developed rat monoclonal antibodies targeting specific immunoglobulin lambda chains. These antibodies are versatile tools for detecting immunoglobulins across various biological samples and formats.
Area of Science:
- Immunology
- Biochemistry
Background:
- Monoclonal antibodies are crucial tools in biological research and diagnostics.
- Specific antibodies targeting immunoglobulin chains are needed for precise detection and characterization.
Purpose of the Study:
- To develop and characterize novel rat monoclonal antibodies against BALB/c myeloma protein M315 (alpha,lambda 2).
- To evaluate the utility of these antibodies in various immunological detection methods.
Main Methods:
- Production of rat monoclonal antibodies against M315 myeloma protein.
- Characterization of antibody binding specificities (V and C domains, cross-reactivity).
- Application of antibodies in radioimmunoassay, ELISA, immunoblotting, and immunofluorescence.
Main Results:
- Two anti-lambda monoclonal antibodies, 9A8 (IgG1) and 2B6 (IgG2a), were generated.
- 9A8 binds the V domain of lambda 2, cross-reacting with lambda 1 and 3; 2B6 targets the C domain of lambda 2, cross-reacting with C lambda 3.
- Antibodies detect immunoglobulins in various forms (soluble, immobilized, blotted, cell-surface).
- A C alpha 2/C alpha 3-specific monoclonal antibody, 8D2 (IgG2a), was also described.
Conclusions:
- The developed monoclonal antibodies are specific and versatile reagents for detecting immunoglobulin lambda chains.
- These antibodies can be readily purified and modified (biotinylated, conjugated) without losing binding capacity.
- The antibodies are valuable tools for diverse immunological assays and research applications.