Related Experiment Video
Updated: Apr 27, 2026

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
Published on: September 22, 2011
Expression of sphingosine 1-phosphate 1-3 on penile cavernous tissue in hypertensive and normotensive rats
Boyi Wang1, Jun Jiang2, Zhongcai Fan1
1Department of Cardiovascular Diseases, Affiliated Hospital, Luzhou Medical College, Luzhou, China.
Objective:
To investigate the expression of sphingosine 1-phosphate 1-3 (S1P1-3) on penile cavernous tissues in hypertensive and normotensive rats and its association with erectile function.
Methods:
Ten 14-week-old male spontaneously hypertensive rats (SHRs) and 10 male age-matched normotensive Wistar-Kyoto rats were randomly designated as hypertensive rat group and normotensive control group, respectively. Mean blood pressure, serum testosterone, and maximum intracavernosal pressure/mean arterial pressure (ICPmax-to-MAP ratio) were measured. The expression of S1P1-3, endothelial nitric oxide synthase (eNOS), phosphorylated eNOS (P-eNOS), and rho-associated protein kinase 1-2 (ROCK1-2) was determined in the cavernous tissues of SHRs and Wistar-Kyoto rats by the immunohistochemical study and Western blot.
Results:
There was no significant difference in body weight and serum testosterone levels between the 2 groups. The MAP in the hypertensive rat group was significantly increased compared with the control group. After 3- and 5-V electrostimulation, the ICPmax-to-MAP ratio in the control group was significantly increased compared with the hypertensive rat group. The protein expression of eNOS, P-eNOS, and S1P1 was significantly higher in the control group than in the hypertensive rat group (P <.05). The protein expression of S1P2, S1P3, and ROCK1-2 was significantly lower in the normotensive control group than in the hypertensive rat group (P <.05).
Conclusion:
Hypertension may impair the erectile function by means of downregulating the expression of S1P1 and upregulating the expression of S1P2-3 in cavernous tissues of SHRs.
Related Concept Videos
Nitric Oxide Signaling Pathway
Antihypertensive Drugs: Vasodilators
Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
Male Sexual Response: Erection & Ejaculation
The blood filling the erectile tissues compresses the veins, which helps to prevent blood from leaving...
Hypertension II: Pathophysiology

