Related Experiment Video
Updated: Apr 27, 2026
10:47
Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
Published on: May 5, 2023
4.1K
Complete mitochondrial genome of the Trachypithecus cristatus
Qin-yang Jiang1, Yan-na Huang1
1a College of animal science and technology, Guangxi University , Nanning , China.
Summary
The complete mitochondrial genome of the Javan langur (Trachypithecus cristatus) was sequenced. Its structure is similar to other vertebrates, offering insights for future phylogenetic research.
Area of Science:
- Genomics
- Molecular Biology
- Zoology
Background:
- Mitochondrial genomes are crucial for understanding evolutionary relationships.
- Trachypithecus cristatus, the Javan langur, is a primate species with limited genomic data available.
- Previous studies on primate mitochondrial DNA have established conserved structural patterns.
Purpose of the Study:
- To determine the complete mitochondrial genome sequence of Trachypithecus cristatus.
- To analyze the structural organization and gene content of the T. cristatus mitochondrial genome.
- To provide foundational data for phylogenetic analyses of Trachypithecus species.
Main Methods:
- DNA extraction from Trachypithecus cristatus samples.
- Polymerase Chain Reaction (PCR) amplification of mitochondrial DNA fragments.
- Genome sequencing and assembly.
Main Results:
- The complete mitochondrial genome of Trachypithecus cristatus was successfully sequenced.
- The genome is 16,557 base pairs in length.
- It comprises 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region.
- The gene order and structural organization are conserved and similar to those observed in most other vertebrates.
Conclusions:
- The determined mitochondrial genome sequence provides a valuable resource for Trachypithecus cristatus.
- The conserved genomic structure supports its utility in comparative genomics.
- This data will facilitate future phylogenetic studies investigating the evolutionary history of langurs and related primates.
Related Concept Videos
02:59Animal Mitochondrial Genetics
7.8K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.8K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
11.5K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
11.5K
02:19Export of Mitochondrial and Chloroplast Genes
3.1K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.1K
01:28The Inner Mitochondrial Membrane
3.8K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.8K
01:39Mitochondrial Protein Sorting
4.4K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.4K
01:37Mitochondria
13.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
13.4K