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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Related Experiment Video

Updated: Apr 27, 2026

Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid

Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid

Published on: June 17, 2025

Punctate pemphigus: an underreported direct immunofluorescence pattern

Gilles F H Diercks¹, Marcel F Jonkman, Hendri H Pas

¹Centre for Blistering Diseases, Department of Dermatology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands; Department of Pathology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.

Journal of Cutaneous Pathology

|July 1, 2014

Summary

No abstract available in PubMed .

Keywords:

dermato pathology desmosome pemphigus

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