[Investigations on the standardization of the lysoplate technique for assay of lysozyme]

In this paper studies for the standardization of the estimation of lysozyme in low concentrations concerning the lysoplate technique are reported. The influence of the conditions of diffusion, the concentration and the age of the bacteria as well as the judgement of different pictures of lysis and contrast resp. have been measured and compared. Sufficient results have been obtained with "Purified agar" (Difco) in a concentration of 0.9% and with living, 48 h old strains from Micrococcus luteus. After this a mixture of the bacteria in a concentration of 2.75 x 10(8)/ml with 5 ml "Purified agar" at 60 degrees C can be recommended. Using conditions of incubation with 50 degrees C and a final time of 4 h lysozyme can be estimated certainly with a sensitivity of 1 microgram/ml.