Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.1K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same author

Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.

APL photonics·2026
Same author

Exercise Training Stimulates the Release of Glutathione Peroxidase 1 (GPX1)-Enriched Extracellular Vesicles That Promote Angiogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Microblasting Wound Dressings Mechanically Disrupt Polymicrobial Biofilms to Enhance Healing in Treatment-Resistant Wounds.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Surface versus Nanocatalyst-Induced Matrix Bubbles Govern Temperature-Dependent Biofilm Removal.

ACS applied materials & interfaces·2026
Same author

Investigating Cellular Magnetic Bioeffects Using Two-Channel, Two-Photon Autofluorescence Lifetime Microscopy.

IEEE transactions on molecular, biological, and multi-scale communications·2026

Related Experiment Video

Updated: Apr 27, 2026

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
12:27

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

Published on: November 25, 2009

8.9K

Multifocal interferometric synthetic aperture microscopy.

Yang Xu, Xiong Kai Benjamin Chng, Steven G Adie

    Optics Express
    |July 1, 2014
    PubMed
    Summary
    This summary is machine-generated.

    We introduce Multifocal Interferometric Synthetic Aperture Microscopy (MISAM), a novel optical coherence tomography method. MISAM overcomes resolution-depth trade-offs for enhanced imaging in various applications.

    More Related Videos

    A Multimodal Wide-Field Fourier-Transform Raman Microscope
    06:48

    A Multimodal Wide-Field Fourier-Transform Raman Microscope

    Published on: December 30, 2025

    858
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    9.4K

    Related Experiment Videos

    Last Updated: Apr 27, 2026

    Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
    12:27

    Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

    Published on: November 25, 2009

    8.9K
    A Multimodal Wide-Field Fourier-Transform Raman Microscope
    06:48

    A Multimodal Wide-Field Fourier-Transform Raman Microscope

    Published on: December 30, 2025

    858
    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    9.4K

    Area of Science:

    • Biomedical Optics
    • Microscopy
    • Optical Imaging

    Background:

    • Optical coherence tomography (OCT) faces a fundamental trade-off between transverse resolution and depth of field (DOF).
    • Multifocal OCT and Interferometric Synthetic Aperture Microscopy (ISAM) offer separate solutions to this limitation.
    • Existing methods require experimental modification or complex post-processing.

    Purpose of the Study:

    • To develop a unified approach combining the strengths of multifocal OCT and ISAM.
    • To address the inverse problem of multifocal OCT.
    • To present a general algorithm for combining multiple ISAM datasets.

    Main Methods:

    • Developed a regularized combination algorithm for resampled ISAM datasets.
    • Integrated multifocal imaging principles with ISAM post-processing.
    • Validated the Multifocal ISAM (MISAM) approach through theory, simulations, and experimental data.

    Main Results:

    • Achieved optimal transverse resolution and an extended effective DOF.
    • Demonstrated significant improvement in signal-to-noise ratio.
    • Successfully combined advantages of multifocal OCT and ISAM in a single framework.

    Conclusions:

    • MISAM offers a powerful solution to the resolution-DOF trade-off in OCT.
    • The developed algorithm provides a versatile tool for advanced optical imaging.
    • MISAM enhances imaging capabilities for demanding applications.