Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyene--sterol interaction and selective toxicity.

C M Gary-Bobo1

  • 1Service de Biophysique, Département de Biologie, Gif-Sur-Yvette, France.

Biochimie
|January 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kinetic study of interaction between [14C]amphotericin B derivatives and human erythrocytes: relationship between binding and induced K+ leak.

Biochimica et biophysica acta·1990
Same author

Relationship between ionophoric and haemolytic activities of perimycin A and vacidin A, two polyene macrolide antifungal antibiotics.

Biochemical pharmacology·1989
Same author

Amphotericin B-sterol complex formation and competition with egg phosphatidylcholine: a monolayer study.

Biochimica et biophysica acta·1988
Same author

Interaction of 14C-labelled amphotericin B derivatives with human erythrocytes: relationship between binding and induced K+ leak.

Biochimica et biophysica acta·1988
Same author

Effect of the polar head structure of polyene macrolide antifungal antibiotics on the mode of permeabilization of ergosterol- and cholesterol-containing lipidic vesicles studied by 31P-NMR.

Molecular pharmacology·1986
Same author

Transfer of amphotericin B from gel state vesicles to mycoplasma cells: biphasic action on potassium transport and permeability.

Antimicrobial agents and chemotherapy·1985
Same journal

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Biochimie·2026
Same journal

Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

Biochimie·2026
Same journal

Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

Biochimie·2026
Same journal

Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

Biochimie·2026
Same journal

Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

Biochimie·2026
Same journal

Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

Biochimie·2026
See all related articles

Amphotericin B derivatives

Area of Science:

  • Biochemistry
  • Membrane Biophysics
  • Medicinal Chemistry

Background:

  • Amphotericin B is a polyene antifungal agent that interacts with sterols in cell membranes.
  • Understanding the structure-activity relationship of amphotericin B derivatives is crucial for developing more targeted therapies.

Purpose of the Study:

  • To investigate how modifications to the carboxyl group of amphotericin B affect its membrane permeability and sterol interaction.
  • To elucidate the mechanism of action of different amphotericin B derivatives in biological and model membranes.

Main Methods:

  • Permeability experiments using biological (erythrocyte) and model (lipidic vesicle) membranes.
  • Binding measurements to assess the partitioning of derivatives within membranes.
  • Comparative analysis of derivatives with free versus blocked carboxyl groups.

Related Experiment Videos

Main Results:

  • Derivatives with a blocked carboxyl group selectively increase permeability in ergosterol-rich membranes, unlike those with a free carboxyl group, which are equally effective in cholesterol and ergosterol membranes.
  • No correlation was found between derivative binding affinity and their ability to induce membrane permeability.
  • Blocked carboxyl derivatives form 'channel' type pores in ergosterol-containing membranes, while free carboxyl derivatives form 'channel' type pores irrespective of sterol type.

Conclusions:

  • The carboxyl group's status (free or blocked) dictates the sterol specificity and mechanism of membrane permeabilization by amphotericin B derivatives.
  • A model for polyene-sterol interaction is proposed, explaining the observed differences in membrane permeability based on derivative structure and sterol composition.