Related Experiment Video

Updated: Apr 27, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

796

Phenotypic characteristics of diabetic kidney involvement

Roland C Blantz1

  • 1Division of Nephrology-Hypertension, University of California, San Diego School of Medicine, and Veterans Affairs San Diego Healthcare System, San Diego, California, USA.

Kidney International
|July 1, 2014
PubMed

Insights

Diabetic kidneys exhibit metabolic changes, including increased oxygen use and hypoxia. Reducing oxygen intake in diabetic kidneys shifts metabolism towards the Warburg effect, unlike in healthy kidneys.

Area of Science:

  • Nephrology
  • Metabolic research
  • Diabetic complications

Background:

  • Diabetic nephropathy is a major complication of diabetes.
  • Understanding kidney metabolic changes is crucial for developing new therapies.
  • Diabetic kidneys show glomerular hyperfiltration, growth, and metabolic abnormalities.

Purpose of the Study:

  • To investigate the metabolic characteristics of the diabetic kidney.
  • To understand the kidney's response to reduced oxygen availability in diabetes.
  • To identify potential shifts in cellular metabolism under diabetic conditions.

Main Methods:

  • Inducing hypoxia by decreasing inspired oxygen to 10% in diabetic and control subjects.
  • Measuring partial pressure of oxygen (pO2), oxygen consumption, lactate levels, and redox potential.
  • Analyzing metabolic shifts, specifically looking for evidence of Warburg metabolism.

Main Results:

  • Diabetic kidneys exhibit increased oxygen consumption leading to hypoxia.
  • Reducing inspired oxygen exacerbated hypoxia and increased lactate in diabetic kidneys.
  • A significant shift towards Warburg metabolism was observed exclusively in the diabetic kidney under hypoxic conditions.

Conclusions:

  • The diabetic kidney has distinct metabolic vulnerabilities.
  • Hypoxia in diabetic kidneys promotes a shift to Warburg metabolism.
  • Targeting these metabolic pathways could offer new therapeutic strategies for diabetic nephropathy.

More Related Videos

Whole-Kidney Three-Dimensional Staining with CUBIC04:31

Whole-Kidney Three-Dimensional Staining with CUBIC

Published on: July 18, 2022

3.9K
Assessment of Kidney Function in Mouse Models of Glomerular Disease09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

21.0K

Related Concept Videos

Diabetic Nephropathy01:28

Diabetic Nephropathy

Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration...
28
Type I Diabetes III: Clinical Manifestations01:19

Type I Diabetes III: Clinical Manifestations

Type 1 diabetes mellitus typically presents with rapid-onset symptoms due to the body’s inability to utilize glucose in the absence of insulin. Since insulin is required for glucose uptake into cells, its deficiency leads to hyperglycemia and cellular energy deprivation, resulting in characteristic clinical features.Polyuria and PolydipsiaOne of the earliest, most prominent symptoms is polyuria (excessive urination). When blood glucose concentrations rise above the renal threshold, the...
21
Diabetic Retinopathy01:27

Diabetic Retinopathy

DefinitionDiabetic retinopathy is a microvascular complication of diabetes affecting the retinal blood vessels.Risk FactorsDiabetic retinopathy is present in almost all individuals with type 1 diabetes and more than 60% of those with type 2 diabetes after two decades of disease.The risk increases with poor glycemic control, hypertension, dyslipidemia, smoking, pregnancy, and puberty.Although cataracts and glaucoma are also more frequent in people with diabetes, retinopathy remains the leading...
51
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
1.1K
Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
1.3K
Diabetic Ketoacidosis ll: Pathophysiology01:22

Diabetic Ketoacidosis ll: Pathophysiology

Diabetic ketoacidosis (DKA) is a metabolic emergency characterized by hyperglycemia, ketonemia, and metabolic acidosis. It results from severe insulin deficiency and an excess of counterregulatory hormones, leading to uncontrolled lipolysis, ketogenesis, and widespread electrolyte and fluid disturbances.Pathophysiology The central event in DKA is a profound loss of insulin action. Without insulin, glucose uptake in insulin-dependent tissues is impaired, while hepatic glucose production...
19