Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mycosynthesis of Zinc oxide nanoparticles using endophytic fungi isolated from <i>Buchanania</i> sp. and its assessment as nanobiopotential.

3 Biotech·2026

Same author

Species-Specific Optimisation and Environmental Regulation of Biofilm Formation in Enterobacter cloacae: Inhibitory Role of Glucose in Biofilm Development.

Environmental microbiology reports·2026

Same author

Retraction Note: Re-engineered BCG overexpressing cyclic di-AMP augments trained immunity and exhibits improved efficacy against bladder cancer.

Nature communications·2026

Same author

Development and validation of the Hypersensitivity Pneumonitis South Asian Questionnaire for Exposure (HP-SAQE): a novel, qualitative and quantitative tool.

Thorax·2026

Same author

Subclinical atrial fibrillation: Implications of recent trials for guideline updates?

World journal of cardiology·2026

Same author

Coronary drug-coated balloons: Current evidence and emerging trends.

World journal of cardiology·2026

Search research articles

Related Experiment Video

Updated: Apr 27, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Quantitative phase imaging using a deep UV LED source.

Alok Kumar Singh, Ahmad Faridian, Peng Gao

|July 1, 2014

Summary

This summary is machine-generated.

We developed a simple, cost-effective method for high-resolution phase imaging using deep ultraviolet (DUV) LED light. This technique achieves high contrast and resolution in biological and non-biological samples.

More Related Videos

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Apr 27, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons

Published on: October 8, 2014

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

Area of Science:

Optics and Photonics
Biomedical Imaging
Materials Science

Background:

Phase imaging is crucial for visualizing transparent specimens.
Traditional phase contrast methods often require complex setups or specific light sources.
Deep ultraviolet (DUV) light offers potential for enhanced imaging due to its short wavelength.

Purpose of the Study:

To present a novel, high-resolution phase imaging method.
To utilize an incoherent deep ultraviolet (DUV) light-emitting diode (LED) source.
To achieve cost-effective and simple phase imaging for diverse samples.

Main Methods:

Recording diffraction patterns at various axial planes.
Retrieving phase information via angular spectrum propagation.
Employing an incoherent DUV LED source (285 nm) without coherence-enhancing filters.

Main Results:

Demonstrated high-resolution phase imaging of biological and non-biological samples.
Achieved simultaneous high resolution and high contrast.
Validated the effectiveness of the DUV LED source for phase imaging.

Conclusions:

The proposed method offers a simple, cost-effective solution for high-resolution phase imaging.
The use of incoherent DUV light provides excellent contrast and resolution, especially for biological samples.
This technique has potential applications in microscopy and materials characterization.