Related Experiment Video
Updated: Apr 27, 2026
14:10
Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
Published on: July 29, 2022
5.0K
Dynamic changes in thrombin generation in abdominal sepsis in mice
Yongzhi Wang1, Oscar O Braun, Su Zhang
1*Department of Clinical Sciences, Malmö, Section of Surgery, †Department of Clinical Sciences, Lund, Section for Cardiology, and ‡Department of Laboratory Medicine, Malmö, Section of Clinical Chemistry, Lund University, Sweden.
Shock (Augusta, Ga.)
|July 1, 2014
Summary
Systemic inflammatory response syndrome and severe infections disrupt coagulation. This study shows abdominal sepsis initially boosts thrombin generation, then significantly reduces it by decreasing key coagulation factors and increasing activated protein C.
Area of Science:
- Coagulation and Thrombosis
- Sepsis Pathophysiology
- Inflammatory Response
Background:
- Severe infections and systemic inflammatory response syndrome (SIRS) profoundly impact the coagulation system.
- Understanding dynamic changes in coagulation during sepsis is crucial for managing hemostatic complications.
Purpose of the Study:
- To investigate the temporal alterations in thrombin generation during experimental abdominal sepsis.
- To elucidate the underlying mechanisms, including changes in coagulation factors and activated protein C levels.
Main Methods:
- Abdominal sepsis was induced in mice using cecal ligation and puncture (CLP).
- Thrombin generation, coagulation factor levels, and activated protein C were measured at 1, 3, 6, and 24 hours post-CLP.
- Histological analysis and myeloperoxidase activity assessed lung inflammation and damage.
Main Results:
- CLP induced a systemic inflammatory response, characterized by neutrophil infiltration, tissue damage, thrombocytopenia, and leukocytopenia.
- Thrombin generation initially increased at 1 hour post-CLP, followed by a marked decrease at 6 and 24 hours.
- Sepsis led to decreased plasma levels of prothrombin, factor V, and factor X, and increased activated protein C at 6 hours.
Conclusions:
- Abdominal sepsis exhibits a biphasic effect on thrombin generation: initial increase followed by significant reduction.
- The sepsis-induced decrease in thrombin generation is linked to reduced coagulation factor levels and elevated activated protein C.
- These findings provide insights into the complex hemostatic derangements observed in severe sepsis.
