Related Experiment Video
Updated: Apr 27, 2026

Induction of Paralysis and Visual System Injury in Mice by T Cells Specific for Neuromyelitis Optica Autoantigen Aquaporin-4
Published on: August 21, 2017
Enhanced expression of aquaporin 9 in activated polymorphonuclear leukocytes in patients with systemic inflammatory
Asako Matsushima1, Hiroshi Ogura, Taichin Koh
1*Critical Care and Trauma Center, Osaka General Medical Center; †Department of Traumatology and Acute Critical Medicine, Osaka University Graduate School of Medicine; and ‡Hoshigaoka Koseinenkin Hospital, Osaka, Japan.
Abstract:
Aquaporins (AQPs) are water channels of cell membranes. All living cells experience osmotic pressure changes in their environment, but the mechanism by which water influx occurs was not known until the discovery of AQPs. AQP9, which is expressed in human polymorphonuclear leukocytes (PMNLs), is reported to relate to morphologic changes of PMNLs in vitro. We examined the expression of AQP9 in PMNLs from patients with systemic inflammatory response syndrome (SIRS) and addressed the role of AQP9 in both morphologic and functional changes of PMNLs in the SIRS condition. Fourteen patients with SIRS were included in our study. Polyclonal antibody was used for the AQP9 assay. F-actin polymerization, oxidative activity, and the expression of AQP9 in PMNLs with and without stimulation by N-formylmethionyl-leucyl-phenylalanine were evaluated by flow cytometry. Expression of AQP9, F-actin polymerization, and oxidative activity in PMNLs were increased significantly in patients with SIRS compared with those in healthy volunteers. The time course of AQP9 fluorescence in PMNLs corresponded to the time course of F-actin polymerization, which showed peak fluorescence at 1 min after N-formylmethionyl-leucyl-phenylalanine stimulation. The expression of AQP9 in PMNLs is increased significantly in SIRS patients. The increased expression of AQP9 in SIRS patients might be associated with F-actin polymerization in PMNLs, which could affect both morphologic and functional changes of PMNLs in the SIRS condition.
Related Concept Videos
Acute Inflammation II: Local and Systemic Effects
Aquaporins
Inflammatory Response I: Vascular and Cellular
Acute Inflammation I: Inflammatory Response
Acute Inflammation I: Cellular Phase
Inflammation

