Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CD38-Targeted Theranostics of Lymphoma with <sup>89</sup>Zr/<sup>177</sup>Lu-Labeled Daratumumab.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2021

Same author

Ultrasmall Porous Silica Nanoparticles with Enhanced Pharmacokinetics for Cancer Theranostics.

Nano letters·2021

Same author

In vivo characterization of PD-L1 expression in breast cancer by immuno-PET with <sup>89</sup>Zr-labeled avelumab.

American journal of translational research·2020

Same author

<sup>86/90</sup>Y-Labeled Monoclonal Antibody Targeting Tissue Factor for Pancreatic Cancer Theranostics.

Molecular pharmaceutics·2020

Same author

A Melanin-Based Natural Antioxidant Defense Nanosystem for Theranostic Application in Acute Kidney Injury.

Advanced functional materials·2020

Same author

ImmunoPET imaging of CD38 expression in hepatocellular carcinoma using <sup>64</sup>Cu-labeled daratumumab.

American journal of translational research·2019

Search research articles

Related Experiment Video

Updated: Apr 27, 2026

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Published on: November 20, 2018

Intrinsically radiolabeled nanoparticles: an emerging paradigm.

Shreya Goel¹, Feng Chen, Emily B Ehlerding

¹Materials Science Program, University of Wisconsin-Madison, 1509 University Avenue Madison, WI, 53706-15952, USA.

Small (Weinheim an Der Bergstrasse, Germany)

|July 1, 2014

Summary

Intrinsically radiolabeled nanoparticles offer a simpler, faster alternative to traditional radiolabeling methods. This emerging technique shows promise for next-generation molecular imaging applications.

Keywords:

Intrinsic radiolabeling chelator-free multimodality imaging nanomaterials positron emission tomography single photon emission computed tomography

More Related Videos

A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines

A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines

Published on: March 4, 2017

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Published on: October 10, 2016

Related Experiment Videos

Last Updated: Apr 27, 2026

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /T1Magnetic Resonance Imaging

Published on: November 20, 2018

A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines

A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines

Published on: March 4, 2017

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Published on: October 10, 2016

Area of Science:

Nanotechnology
Radiochemistry
Molecular Imaging

Background:

Traditional chelator-based radiolabeling faces challenges including complex chemistry and potential radioisotope detachment.
Existing methods can alter carrier pharmacokinetics, impacting imaging accuracy.

Purpose of the Study:

To introduce intrinsically radiolabeled nanoparticles as a novel radiolabeling technique.
To highlight their potential for simplified and improved molecular imaging.

Main Methods:

Synthesis of intrinsically radiolabeled nanoparticles using methods like hot-plus-cold precursors, specific trapping, cation exchange, and proton beam activation.
Application of these nanoparticles in multimodality molecular imaging.

Main Results:

Demonstrated potential for easier, faster, and more specific radiolabeling compared to conventional methods.
Showcased multifunctional nanoparticles for advanced molecular imaging.

Conclusions:

Intrinsically radiolabeled nanoparticles represent a promising advancement in radiolabeling technology.
This approach offers significant potential for the next generation of molecular imaging, despite being in early stages.