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Related Experiment Videos

Conserved gene structures and expression signals in methanogenic archaebacteria.

R Allmansberger1, M Bokranz, L Kröckel

  • 1Department of Biology, Philipps University, Marburg, Federal Republic of Germany.

Canadian Journal of Microbiology
|January 1, 1989
PubMed
Summary

Comparative analysis of methyl coenzyme M reductase (mcr) genes in methanogens reveals varying conservation. Archaebacterial mcr genes show greater homology to eukaryotic than eubacterial genes, suggesting evolutionary gene fusion.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Investigated cotranscribed gene clusters of methyl coenzyme M reductase (mcr) structural genes (mcrA, mcrB, mcrC, mcrD, mcrG) in three methanogen species.
  • mcrA, mcrB, and mcrG encode subunits of methyl coenzyme M reductase; the functions of mcrC and mcrD remain unknown.

Purpose of the Study:

  • To comparatively analyze the conservation of mcr gene products across different methanogen species.
  • To investigate evolutionary relationships by comparing mcr genes and RNA polymerase genes with prokaryotic and eukaryotic homologs.
  • To identify conserved DNA elements associated with mcr gene transcription.

Main Methods:

  • Comparative sequence analysis of mcr gene clusters in methanogens.
  • Analysis of RNA polymerase core subunit genes from Methanobacterium thermoautotrophicum and comparison with Escherichia coli, Saccharomyces cerevisiae, and Drosophila melanogaster.

Related Experiment Videos

  • Examination of G+C content and amino acid sequence conservation.
  • Identification of ribosome binding sites and conserved DNA elements near transcription initiation sites.

    • Main Results:

    • Variable conservation of mcr gene products was observed among methanogen species.
    • No correlation was found between G+C content conservation and amino acid sequence conservation of homologous genes.
    • Homologous polypeptide domains in RNA polymerase genes suggest gene fusion events during evolution.
    • Archaebacterial mcr subunits exhibit stronger homology to eukaryotic than eubacterial equivalents at the amino acid sequence level.
    • Eubacterial-type ribosome binding sites precede all analyzed genes.
    • Conserved structural DNA elements were detected near mcr gene transcription start sites.

    Conclusions:

    • The evolutionary history of mcr genes involves varying degrees of conservation and potential gene fusion events.
    • Archaebacterial genes share closer evolutionary ties with eukaryotes than with eubacteria.
    • Conserved regulatory elements likely play a role in the transcription of mcr genes in methanogens.