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Invisible RNA state dynamically couples distant motifs.

Janghyun Lee1, Elizabeth A Dethoff2, Hashim M Al-Hashimi3

  • 1Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055;

Proceedings of the National Academy of Sciences of the United States of America
|July 1, 2014
PubMed
Summary
This summary is machine-generated.

The HIV-1 transactivation response element RNA dynamically shifts to a transient state, altering its structure and creating new base pairs. This reveals RNA

Keywords:
NMR spectroscopyR1ρ relaxation dispersiondynamicsnucleic acids

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Area of Science:

  • Molecular Biology
  • Biophysics
  • Structural Biology

Background:

  • The HIV-1 transactivation response element (TAR) RNA is crucial for viral replication.
  • Understanding the dynamic structural transitions of TAR RNA is essential for its function.

Purpose of the Study:

  • To investigate the dynamic equilibrium and structural remodeling of HIV-1 TAR RNA.
  • To characterize the transient states involved in RNA structural dynamics.

Main Methods:

  • Utilized on- and off-resonance carbon and nitrogen R1ρ NMR relaxation dispersion.
  • Employed mutagenesis and NMR chemical shift fingerprinting techniques.

Main Results:

  • Identified a transient state of TAR RNA with a lifetime of ~2 ms and population of ~0.4%.
  • Observed simultaneous remodeling of bulge, stem, and apical loop structures.
  • Demonstrated a global change in strand register leading to noncanonical base pair formation.

Conclusions:

  • Transient RNA states can induce significant structural changes in distant motifs.
  • These dynamics may facilitate rapid long-range communication in RNA.
  • Findings offer insights into cooperative folding and ribonucleoprotein assembly mechanisms.