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Updated: Apr 27, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Miniaturization: Chip-based liquid chromatography and proteomics
Georges L Gauthier1, Rudolf Grimm2
1Agilent Technologies R&D and Marketing GmbH & Co. KG, 76337 Waldbronn, Germany.
Abstract:
Proteomic research is linked with significant technological challenges such as the high dynamic range and low abundance of biologically interesting proteins. Moreover, there is an increasing need for high-throughput and robustness of routinely performed analyses. Solving these difficulties requires refinements in the capability to fractionate and prepare biological samples as well as improvements in speed, automation, separation power and overall analytical sensitivity.Recent innovations in microfluidic devices with integrated on-chip sample enrichment, liquid chromatography and electrospray emitters and their applicability for specific proteomic applications are presented in this review.:
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