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Related Experiment Videos

Minimum eliciting patch test concentrations of cobalt.

C F Allenby1, D A Basketter

  • 1Lister Hospital, Stevenage, Hertfordshire, UK.

Contact Dermatitis
|March 1, 1989
PubMed
Summary

This study investigated cobalt allergy by testing patch test concentrations in sensitized individuals. Surfactant pretreatment enhanced skin reactivity to cobalt chloride, lowering the minimum eliciting concentration.

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Area of Science:

  • Allergy and Immunology
  • Dermatology
  • Toxicology

Background:

  • Cobalt chloride is a known allergen.
  • Assessing the minimum eliciting concentration (MEC) is crucial for diagnosing cobalt allergy.
  • Skin barrier integrity influences allergic contact dermatitis reactions.

Purpose of the Study:

  • To determine the minimum eliciting levels of cobalt chloride in sensitized individuals.
  • To evaluate the effect of skin pretreatment on cobalt reactivity.
  • To investigate the role of EDTA in modulating cobalt-induced skin reactions.

Main Methods:

  • Patch testing with aqueous cobalt chloride on normal and pretreated skin.
  • Utilized varying concentrations of cobalt chloride (1 ppm to 10,000 ppm).
  • Employed surfactant pretreatment to enhance skin penetration and reactivity.

Main Results:

  • In sensitized subjects, 6 reacted to 10,000 ppm and 1 showed an equivocal reaction to 1000 ppm cobalt chloride.
  • Surfactant pretreatment significantly reduced the MEC, with reactions observed at concentrations as low as 10 ppm in some subjects.
  • Ethylenediaminetetraacetic acid (EDTA) was found to reduce the response to aqueous cobalt.

Conclusions:

  • Skin pretreatment can enhance reactivity to cobalt chloride, lowering the MEC in sensitized individuals.
  • These findings have implications for diagnostic patch testing protocols for cobalt allergy.
  • EDTA may play a role in mitigating cobalt-induced allergic reactions.

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