Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

800.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
800.5K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

833
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
833
DNA Microarrays02:34

DNA Microarrays

16.7K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.7K
Next-generation Sequencing03:00

Next-generation Sequencing

87.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.7K
DNA Isolation01:24

DNA Isolation

35.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
35.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The HEV capsid through a dynamic lens: parallels and divergence from caliciviruses.

Current opinion in virology·2026
Same author

Early intervention with secukinumab prevents epigenetic scar development in new-onset psoriasis: STEPIn mechanistic substudy results.

The Journal of allergy and clinical immunology·2026
Same author

Does Checklist-Guided Shared Decision Making Have a Sustained Effect on Code Status Decisions Among Medical Inpatients? Long-Term Follow Up of the Randomized CLEAR Checklist Trial.

Journal of general internal medicine·2026
Same author

AMIGO - Guided assignment of <sup>13</sup>C-methyl labelled proteins.

Journal of biomolecular NMR·2026
Same author

Oral splicing modulator branaplam in Huntington's disease: a phase 2 randomized controlled trial.

Nature medicine·2026
Same author

Pharmacogenomic, pharmacokinetic, and safety analysis of CYP3A4/CYP3A5 polymorphisms of midostaurin in patients with acute myeloid leukemia.

European journal of clinical pharmacology·2025

Related Experiment Video

Updated: Apr 27, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

11.4K

Current methods for high-throughput detection of novel DNA polymorphisms.

Thomas Peters1, Reinhard Sedlmeier1

  • 1Ingenium Pharmaceuticals AG, Fraunhoferstr. 13, 82152 Martinsried, Germany.

Drug Discovery Today. Technologies
|July 2, 2014
PubMed
Summary

Reliable DNA sequence variation detection is crucial for genetic research. New technologies for cost-effective identification of genetic polymorphisms will advance disease gene mapping and drug discovery.

More Related Videos

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.1K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

41.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
10:41

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Published on: March 29, 2017

11.4K
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.1K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

41.3K

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Accurate detection of DNA sequence variations is fundamental for diverse biological research, including disease gene identification and protein function studies.
  • Advancements in genetic research necessitate reliable and efficient methodologies for identifying novel genetic polymorphisms.

Purpose of the Study:

  • To highlight the importance of dependable methods for detecting DNA sequence variations.
  • To emphasize the impact of rapid and cost-effective technologies for identifying novel genetic polymorphisms.

Main Methods:

  • Not explicitly detailed in the abstract, but implies the use of molecular biology techniques and bioinformatics for DNA sequencing and analysis.
  • Focuses on the development and application of technologies for genetic polymorphism identification.

Main Results:

  • The abstract does not present specific results but discusses the potential impact of new technologies.
  • Anticipates significant advancements in genetic mapping, drug target discovery, and pharmacogenomics.

Conclusions:

  • Reliable DNA sequence variation detection is essential for progress in genetic research.
  • The development of efficient technologies for identifying genetic polymorphisms will accelerate discoveries in medicine and biology.