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Related Experiment Video

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Multispectral upconversion luminescence intensity ratios for ascertaining the tissue imaging depth.

Kai Liu1, Yu Wang, Xianggui Kong

  • 1State Key Laboratory of Luminescence and Applications, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, P. R. China. xgkong14@ciomp.ac.cn.

Nanoscale
|July 2, 2014
PubMed
Summary

This study introduces a novel method using upconversion nanoparticles (UCNPs) to determine tissue imaging depth. The luminescence intensity ratio of multispectral NaYF4:Yb(3+),Er(3+) UCNPs enables accurate 3D depth measurements in medical optical imaging.

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Area of Science:

  • Nanotechnology
  • Biomedical Optics
  • Materials Science

Background:

  • Upconversion nanoparticles (UCNPs) are advanced contrast agents for deep tissue luminescence imaging.
  • Current UCNP imaging is limited to 2D, lacking depth information crucial for comprehensive analysis.

Purpose of the Study:

  • To develop a method for accurately determining tissue imaging depth using UCNPs.
  • To enhance medical optical imaging from 2D to 3D by incorporating depth information.

Main Methods:

  • A theoretical model was established to quantify the relationship between UCNP luminescence intensity ratios and imaging depth.
  • Multispectral NaYF4:Yb(3+),Er(3+) UCNPs were utilized, monitoring the 540 nm/650 nm intensity ratio (G/R ratio) under excitation (Ex) and emission (Em) path schemes.
  • Tissue-mimicking liquid phantoms and layered pork muscles were used to calibrate and validate the depth measurement model.

Main Results:

  • The G/R ratio of NaYF4:Yb(3+),Er(3+) UCNPs accurately correlates with UCNP imaging depth.
  • The developed model demonstrated high measurement accuracy up to centimeter thickness in validated tissue models.
  • The method successfully transitioned UCNP imaging capabilities from 2D to 3D.

Conclusions:

  • A simple and accurate method for determining tissue imaging depth using UCNP luminescence intensity ratios has been successfully developed.
  • This advancement significantly enhances the utility of UCNPs in medical optical imaging, enabling true 3D visualization.
  • The findings pave the way for more informative nanotechnology-based medical imaging techniques.