Essential enrichment strategies in phosphoproteomics

Reversible protein phosphorylation ranks among the most important post-translational modifications that occur in the cell. Therefore, the characterization of phosphorylation events is a highly relevant albeit challenging task. Significant progress surrounding the biochemical analysis of reversible protein phosphorylation in the past 2 years has led to the development of several new techniques to isolate or enrich phosphopeptides, particularly in large-scale analyses. This review gives an overview of the most frequently used phosphopeptide enrichment methods.: