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Updated: Apr 27, 2026

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
Published on: February 5, 2022
Proteomic analysis of GPI-anchored membrane proteins
Hye Ryung Jung1, Ole Nørregaard Jensen1
1Protein Research Group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230, Odense M, Denmark.
Abstract:
Glycosyl-phosphatidyl-inositol-anchored proteins (GPI-APs) represent a subset of post-translationally modified proteins that are tethered to the outer leaflet of the plasma membrane via a C-terminal GPI anchor. GPI-APs are found in a variety of eukaryote species, from pathogenic microorganisms to humans. GPI-APs confer important cellular functions as receptors, enzymes and scaffolding molecules. Specific enzymes and detergent extraction methods combined with separation technologies and mass spectrometry permit proteomic analysis of GPI-APs from plasma membrane preparations to reveal cell-type specific surface molecules, candidate biomarkers and potential therapeutic targets.:
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