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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Published on: February 7, 2022
Compound precipitation in high-concentration DMSO solutions
Ioana Popa-Burke1, John Russell2
1Sample Management Technologies, Molecular Discovery Research, GlaxoSmithKline, Research Triangle Park, NC, USA Ioana.G.Popa-Burke@gsk.com.
Abstract:
Screening compounds precipitate out of high-concentration DMSO solutions and cause issues in the liquid-handling equipment used to process the samples as well as false-positives and false-negatives in the biological data. This report examines the extent of the precipitation issue, correlation with physico-chemical properties of compounds, and measured concentrations. Possible ways to allow recovery of the precipitated solids are also described. This study also investigated whether particulates were present from the time of initial solubilization in DMSO or whether precipitation occurs during freeze-thaw cycling.
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