Related Experiment Video
Updated: Apr 27, 2026

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Published on: October 23, 2016
The regulation of HanA during heterocyst development in cyanobacterium Anabaena sp. PCC 7120
Jing-Jing Lu1, Lei Shi, Wen-Li Chen
1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
Abstract:
In response to deprivation of combined nitrogen, the filamentous cyanobacterium Anabaena sp. strain PCC 7120 develops heterocyst, which is specifically involved in the nitrogen fixation. In this study, we focused on the regulation of HanA, a histone-like protein, in heterocyst development. Electrophoretic mobility shift assay results showed that NtcA, a global nitrogen regulator necessary for heterocyst differentiation, could bind to two NtcA-binding motifs in the hanA promoter region. qPCR results also showed that NtcA may regulate the expression of hanA. By using the hanA promoter-controlled gfp as a reporter gene and performing western blot we found that the amount of HanA in mature heterocysts was decreased gradually.
Related Concept Videos
Stringent Response in E. coli
Gene Regulation During Sporulation
Other Stress Responses in Bacteria
Allosteric Regulation
Regulation of Bacterial Virulence

