Pseudoruegeria sabulilitoris sp. nov., isolated from seashore sand

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and ovoid or rod-shaped bacterial strain, GJMS-35(T), was isolated from seashore sand at Geoje Island, South Korea. Strain GJMS-35(T) grew optimally at 28-30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJMS-35(T) clustered with type strains of species of the genus Pseudoruegeria, with which it exhibited 96.97-98.42 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of other recognized species were less than 96.39 %. Strain GJMS-35(T) contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GJMS-35(T) were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GJMS-35(T) was 64.1 mol% and its mean DNA-DNA relatedness values with type strains of three species of the genus Pseudoruegeria were 11-21 %. Its differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GJMS-35(T) is set apart from other species of the genus Pseudoruegeria. On the basis of the data presented, strain GJMS-35(T) is considered to represent a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria sabulilitoris sp. nov. is proposed. The type strain is GJMS-35(T) ( = KCTC 42111(T) = NBRC 110380(T)).