Related Experiment Video
Updated: Apr 27, 2026

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
Published on: November 1, 2024
RNA interference as a tool for target validation
1Retrovirology Laboratory IrsiCaixa, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Spain.
Abstract:
RNA interference (RNAi) is a cellular phenomenon, aspects of which still remain partially unknown. Nevertheless, beyond its function in cellular defense or control of development, RNAi provides a powerful tool for the study of gene function and target validation. Here, we review some of the key technologies that have allowed RNAi to be used 'in vitro' and 'in vivo'. We summarize their main advantages and disadvantages, in the hope of creating a basic guideline for scientists needing to introduce RNAi as a tool for basic research.:
Related Concept Videos
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
Experimental RNAi
Homologous Recombination
Improving Translational Accuracy
Improving Translational Accuracy

