Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

10.6K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
10.6K
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

7.3K
7.3K
Complexometric Titration: Ligands00:43

Complexometric Titration: Ligands

2.5K
Different monodentate and polydentate ligands are used as complexing agents in complexometric titration reactions. The formation of complexes by mono- and bidentate ligands involves two or more intermediate steps, limiting their use as complexing agents. In comparison, polydentate ligands can form complexes with metal ions in a single-step process, facilitating sharper end points. This means polydentate ligands, such as amino carboxylic acid derivatives, are most commonly employed in...
2.5K
Potentiometric Titration: Overview01:31

Potentiometric Titration: Overview

5.7K
Potentiometric titration is a quantitative analytical technique that determines the concentration of an analyte by measuring the potential difference between the two electrodes in the solution. The endpoint of a potentiometric titration is the point at which there is a significant change in the potential difference. It occurs when the stoichiometric reaction between the analyte and the titrant is complete. The endpoint is usually determined graphically by plotting the measured potential...
5.7K
Complexometric Titration: Overview00:39

Complexometric Titration: Overview

10.6K
Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free...
10.6K
Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

740
Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
740

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extrapolating differential scanning calorimetry data for monoclonal antibodies to low temperatures.

Analytical biochemistry·2024
Same author

Development of high-affinity nanobodies specific for Na<sub>V</sub>1.4 and Na<sub>V</sub>1.5 voltage-gated sodium channel isoforms.

The Journal of biological chemistry·2022
Same author

Reversibility and irreversibility in the temperature denaturation of monoclonal antibodies.

Analytical biochemistry·2021
Same author

Binding Thermodynamics to Intrinsically Disordered Protein Domains.

Methods in molecular biology (Clifton, N.J.)·2020
Same author

Synthesis of glutamic acid and glutamine peptides possessing a trifluoromethyl ketone group as SARS-CoV 3CL protease inhibitors.

Tetrahedron·2020
Same author

Author Correction: A human monoclonal antibody prevents malaria infection by targeting a new site of vulnerability on the parasite.

Nature medicine·2018

Related Experiment Video

Updated: Apr 27, 2026

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
08:45

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Published on: September 7, 2011

55.9K

Isothermal titration calorimetry: controlling binding forces in lead optimization.

Ernesto Freire1

  • 1Department of Biology, Johns Hopkins University, 3400 North Charles, Baltimore, MD 21218, USA.

Drug Discovery Today. Technologies
|July 2, 2014
PubMed
Summary

Drug discovery faces challenges with similar protein targets. Isothermal titration calorimetry (ITC) is crucial for optimizing drug affinity and selectivity by analyzing binding energy components.

More Related Videos

Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms
10:04

Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms

Published on: April 7, 2011

12.3K
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

2.9K

Related Experiment Videos

Last Updated: Apr 27, 2026

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity
08:45

Isothermal Titration Calorimetry for Measuring Macromolecule-Ligand Affinity

Published on: September 7, 2011

55.9K
Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms
10:04

Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms

Published on: April 7, 2011

12.3K
Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

2.9K

Area of Science:

  • Biochemistry
  • Pharmacology
  • Drug Discovery

Background:

  • The human genome project identified numerous novel drug targets, many belonging to protein families with similar structures and active sites.
  • Developing drugs with high affinity and selectivity against these homologous targets is challenging, necessitating advanced optimization strategies.

Purpose of the Study:

  • To highlight the importance of advanced optimization strategies in drug development.
  • To emphasize the role of isothermal titration calorimetry (ITC) in achieving high affinity and selectivity.

Main Methods:

  • Utilizing isothermal titration calorimetry (ITC) as the primary experimental technique.
  • Analyzing the enthalpic and entropic components of binding energy.

Main Results:

  • ITC provides a unique partition of binding energy into enthalpic and entropic contributions.
  • This detailed analysis aids in understanding and controlling binding forces.

Conclusions:

  • Isothermal titration calorimetry (ITC) is essential for effective lead optimization in drug discovery.
  • ITC enables precise control over forces that enhance target affinity and minimize off-target interactions.