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Does aminoglycoside-acetyltransferase in rapidly growing mycobacteria have a metabolic function in addition to
T Udou1, Y Mizuguchi, R J Wallace
1Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Abstract:
All the rapidly growing mycobacteria tested, Mycobacterium fortuitum complex, M. smegmatis, M. phlei, and M. vaccae, contained one of two characteristics, but were different from previously recognized aminoglycoside-acetyltransferases. The acetylation reaction of both the enzymes from M. fortuitum and Pseudomonas aeruginosa (3-N-acetyltransferase-III) with radiolabeled acetyl coenzyme A was inhibited severely by oxalacetate. It was suggested that the inhibitory effect of oxalacetate is due to the condensation reaction between oxalacetate and acetyl coenzyme A resulting in the generation of citrate.