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Updated: Apr 27, 2026
11:21
Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
Published on: June 26, 2010
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Multiphoton excited fluorescent materials for frequency upconversion emission and fluorescent probes
1Institute of Molecular Functional Materials+, Department of Chemistry and Institute of Advanced Materials, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
Advanced Materials (Deerfield Beach, Fla.)
|July 2, 2014
Summary
Multiphoton absorption (MPA) fluorophores enable advanced applications like frequency upconversion lasing and two-photon excitation (2PA) bioimaging. These technologies offer enhanced spatial resolution and deeper tissue penetration for biological applications.
Area of Science:
- Optics and Photonics
- Biomedical Imaging
- Materials Science
Background:
- Multiphoton absorption (MPA) fluorophores are crucial for advanced optical applications.
- Emerging technologies leverage MPA for frequency upconversion and two-photon excitation (2PA).
Purpose of the Study:
- To review recent progress in MPA fluorophore strategies for specific applications.
- To highlight the advantages of MPA in frequency upconversion lasing and 2PA bioimaging/biosensing.
Main Methods:
- Review of current MPA fluorophore development strategies.
- Analysis of MPA applications in frequency upconversion photoluminescence and lasing.
- Exploration of 2PA fluorescence for bioimaging and biosensing.
Main Results:
- MPA frequency-upconverted lasing enables high-energy blue light sources for laser applications.
- 2PA fluorescence offers significant advantages for biological imaging, including higher resolution and reduced photodamage.
- Versatile 2PA probes are advancing diagnostic and therapeutic treatments.
Conclusions:
- MPA fluorophores are pivotal for breakthroughs in coherent light sources and biomedical applications.
- Further advancements in 2PA probes are anticipated for enhanced bioimaging and biosensing capabilities.
- Key probe characteristics for biological use include specificity, high quantum yield, appropriate wavelengths, stability, solubility, and biocompatibility.
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