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Genome scale cytometry: High content analysis for high throughput RNAi phenotype profiling
David M Evans1, David O Azorsa1, Spyro Mousses1
1Cancer Drug Development Laboratory, Translational Genomics Research Institute, 20, Firstfield Rd, Gaithersburg, MD 20878, USA.
Drug Discovery Today. Technologies
|July 2, 2014
Abstract:
The combination of RNAi-mediated knockdown of gene expression and high content screening (HCS) allows the determination of the contribution of single genes to a variety of cellular effects varying between growth and survival to subtle alterations in cellular morphology and phenotype. This review examines the current status of research in combining these tools.:
