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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

51.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
51.7K

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High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
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High throughput, nanoliter quantitative PCR.

Colin Brenan1, Tom Morrison1

  • 1BioTrove Inc., 12 Gill Street, Suite 4000, Woburn, MA 01810, USA.

Drug Discovery Today. Technologies
|July 2, 2014
PubMed
Summary
This summary is machine-generated.

High throughput quantitative PCR offers accurate gene expression analysis, rivaling microarrays. Miniaturized qPCR is emerging as a powerful tool for large-scale genetic studies.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • The completion of the human genome sequence necessitates advanced methods for analyzing gene expression.
  • Quantitative PCR (qPCR) provides a sensitive alternative to microarrays for gene expression profiling.

Purpose of the Study:

  • To review current research in miniaturized, high throughput quantitative PCR.
  • To assess the potential of qPCR as a viable alternative to microarrays for large-scale genetic analyses.

Main Methods:

  • Review of current scientific literature on miniaturized and high throughput qPCR.
  • Comparative analysis of qPCR and microarray techniques for gene expression analysis.

Main Results:

  • Quantitative PCR demonstrates high accuracy and precision, with sensitivity down to single transcript copies.
  • Miniaturized, high throughput qPCR is rapidly advancing and shows promise for large-scale genetic studies.

Conclusions:

  • Miniaturized, high throughput qPCR is poised to become a leading technique for large-scale genetic analyses.
  • qPCR offers a compelling alternative to hybridization microarrays for quantitative transcription analysis.