Related Experiment Video

Updated: Apr 27, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

1.3K

Molecular epidemiology of NDM-1-producing Enterobacteriaceae and Acinetobacter baumannii isolates from Pakistan

Anna L Sartor1, Muhammad W Raza2, Shahid A Abbasi3

  • 1University of Queensland, UQ Centre for Clinical Research, Herston, Queensland, Australia anna.sartor22@gmail.com.

Antimicrobial Agents and Chemotherapy
|July 2, 2014
PubMed

Insights

Molecular epidemiology revealed clonal spread of NDM-producing Enterobacteriaceae in Pakistani hospitals. Most isolates harbored the IncA/C plasmid group and 16S methylase genes, indicating significant resistance threats.

Area of Science:

  • Molecular biology
  • Epidemiology
  • Antimicrobial resistance

Background:

  • The rise of carbapenemase-producing Enterobacteriaceae (CPE) is a global health concern.
  • New Delhi metallo-β-lactamase (NDM) is a significant carbapenemase, contributing to antimicrobial resistance.

Purpose of the Study:

  • To investigate the molecular epidemiology of NDM-producing isolates from Pakistani hospitals.
  • To determine the genetic relatedness and identify resistance mechanisms in these isolates.

Main Methods:

  • Repetitive sequence-based PCR (Rep-PCR) for genetic relatedness.
  • PCR-based replicon typing for plasmid analysis.
  • Screening for antibiotic resistance genes, including carbapenemases and 16S methylases.

Main Results:

  • Rep-PCR indicated clonal spread of Enterobacter cloacae and Escherichia coli.
  • The IncA/C plasmid group was the most prevalent (78%).
  • 16S methylase-encoding genes were found in 81% of NDM-producing Enterobacteriaceae.

Conclusions:

  • Clonal dissemination of NDM-producing E. cloacae and E. coli is occurring in Pakistani hospitals.
  • Co-harboring of 16S methylase genes on IncA/C plasmids contributes to the spread of multidrug resistance.

More Related Videos

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.8K
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.6K

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
85
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
211
Bacterial Gastroenteritis01:18

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
87