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Telomeres and Telomerase

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Related Experiment Video

Updated: Apr 27, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

A self-regulating template in human telomerase.

Andrew F Brown¹, Joshua D Podlevsky¹, Xiaodong Qi¹

¹Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287.

Proceedings of the National Academy of Sciences of the United States of America

|July 2, 2014

Summary

This summary is machine-generated.

Telomerase RNA (TR) contains a pause signal that ensures accurate DNA repeat synthesis. This mechanism prevents errors and generates precise telomeric DNA products.

Keywords:

polymerase ribonucleoprotein telomeres

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Last Updated: Apr 27, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

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Published on: June 12, 2018

In vitro Reconstitution of the Active T. castaneum Telomerase

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Published on: July 14, 2011

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Estimation of Telomeric Repeat-containing RNA from DNA/RNA Hybrid Complexes

Published on: December 5, 2025

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Telomerase is a reverse transcriptase (RT) synthesizing telomeric DNA using an intrinsic telomerase RNA (TR) template.
The precise mechanism of accurate and efficient telomere repeat synthesis by telomerase has been unclear.

Purpose of the Study:

To elucidate the mechanism by which the human telomerase RNA template guides accurate DNA repeat synthesis.
To investigate the role of sequence-defined pausing in telomere synthesis fidelity.

Main Methods:

Investigated the function of the human telomerase RNA template during DNA synthesis.
Analyzed the impact of a specific sequence-defined pause signal on nucleotide incorporation and product formation.

Main Results:

Identified a single-nucleotide pause signal within the human TR template, triggered after dT incorporation.
Demonstrated that this pause site, coinciding with the P1 boundary, ensures accurate repeat synthesis and prevents off-template incorporation.
Showed that this pausing mechanism is crucial for the characteristic 6-nt banding pattern of telomeric DNA products.

Conclusions:

The human TR template possesses a self-regulating pause mechanism essential for high-fidelity telomere repeat synthesis.
This sequence-defined pausing, alongside the P1 boundary, dictates the precise length and register of synthesized telomeric DNA.