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Coordinated expression of cyclin-dependent kinase-4 and its regulators in human oral tumors

Ming J Poi1, Thomas J Knobloch2, Marta T Sears3

  • 1Department of Pharmacy, The Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, Columbus, OH, U.S.A Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A.

Anticancer Research
|July 2, 2014
PubMed
Abstract

Insights

Aberrant expression of cyclin-dependent kinase-4 (CDK4) and its regulators is common in oral lesions. These oncogenes show coordinated up-regulation in high-risk mucosa and squamous cell carcinoma of the head and neck (SCCHN).

Area of Science:

  • Molecular Oncology
  • Cancer Genomics

Background:

  • Aberrant cyclin-dependent kinase-4 (CDK4) expression is implicated in squamous cell carcinoma of the head and neck (SCCHN).
  • The relationship between CDK4 and its regulators (cyclin D1, cyclin E, gankyrin, SEI1, BMI1) in gene expression within oral tissues requires further investigation.

Purpose of the Study:

  • To investigate the mRNA profiles of CDK4 and its key regulators in various oral lesion tissues.
  • To explore the interrelations among these oncogenes in the context of oral cancer development.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed.
  • Analysis included 30 SCCHN specimens, matched high-risk mucosa (HARM) samples, and 16 healthy control specimens.

Main Results:

  • Significantly elevated mRNA levels of CDK4, cyclin D1, gankyrin, SEI1, and BMI1 were observed in both HARM and SCCHN tissues compared to controls.
  • Statistically significant correlations in gene expression were identified among these markers.

Conclusions:

  • CDK4 and its regulators are coordinately up-regulated during oral cancer progression.
  • High-risk mucosa and SCCHN exhibit a shared molecular signature within the CDK4-pRB pathway.
Keywords:
Cyclin-dependent kinase 4coordinationsquamous cell carcinoma of the head and necktranscription up-regulation

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