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Updated: Apr 27, 2026
Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
Published on: July 23, 2016
Proteinase K-containing lipid nanoparticles for therapeutic delivery of siRNA LOR-1284
Dong Chul Kim1, Young Ah Cho2, Hong Li2
1Department of Pathology, Graduate School of Medicine, Gyeongsang National University, Jinju, South Korea.
Background:
The objective of the present study was to develop an efficient delivery vehicle for siRNA LOR-1284 through incorporation of proteinase K (PrK) as a means of preventing siRNA degradation by serum nucleases. Lipid nanoparticle-PrK-siRNA (LN-PrK-siRNA) complexes were synthesized and characterized.
Materials And Methods:
siRNA complexed with PrK and liposomes composed of dimethyldioctadecyl ammonium bromide/cholesterol/Tween 80 (60:35:5 molar ratio) were investigated for down-regulation of R2 mRNA activity in KB human carcinoma cells.
Results:
Treatment with LN-PrK-siRNA (30:0.3:1 molar ratio) significantly reduced levels of R2 mRNA compared to siRNA-liposomes without PrK in serum-containing medium. LN-PrK-siRNA complexes showed increased stability in serum and reduced toxicity in KB cells relative to LN-siRNA complexes.
Conclusion:
LN-PrK-siRNA complexes are promising delivery vehicles for siRNA.
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