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Updated: Apr 27, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Effect of low-level laser on bone defects treated with bovine or autogenous bone grafts: in vivo study in rat
Mércia J S Cunha1, Luis A Esper2, Michyele C Sbrana2
1Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo, Brazil ; Bauru School of Dentistry, USP, Brazil ; Faculdade de Odontologia de Bauru (FOB), Universidade de São Paulo (USP), Alameda Dr. Octávio Pinheiro Brisolla 9-75, Vila Universitária, 17012 901 Bauru, SP, Brazil.
Objective:
The purpose of this study was to histologically evaluate the effect of low-level laser (LLL) on the healing of critical size defects (CSD) in rat calvaria, filled with autogenous or inorganic bovine bone grafts.
Methods:
Sixty rats were divided into 6 groups (n = 10): C (control-filled with blood clot), LLL (low-level laser-GaAlAs, λ 780 nm, 100 mW, 210 J/cm(2), Φ 0.05 cm(2); 6 J/point), AB (autogenous bone), ABL (autogenous bone + low-level laser), OB (inorganic bovine bone), and OBL (inorganic bovine bone + LLL).
Material And Methods:
The animals were killed after 30 days. Histological and histometric analyses were performed by light microscopy. Results. The groups irradiated with laser, LLL (47.67% ± 8.66%), ABL (39.15% ± 16.72%), and OBL (48.57% ± 28.22%), presented greater area of new bone formation than groups C (9.96% ± 4.50%), AB (30.98% ± 16.59%), and OB (11.36% ± 7.89%), which were not irradiated. Moreover, they were significantly better than group C (Kruskal-Wallis test followed by Dunn test, P < 0.05).
Conclusion:
The laser accelerated the healing of bone defects and the resorption of particles of the graft material.
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