Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.3K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.3K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

992
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
992

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Boosting Boron Neutron Capture Therapy through Ferroptosis Activation with a Biomimetic Nanoenhancer.

Biomaterials research·2026
Same author

Deep Learning Enables Pixel-Level Nanoparticle Distribution Mapping in Routine Histological Sections by Integrating Cancer Associated Fibroblasts Features.

ACS nano·2026
Same author

A study on the climate-driven spatiotemporal dynamics of influenza in Lanzhou spanning the COVID-19 era.

Frontiers in cellular and infection microbiology·2026
Same author

High Dietary Supplementation of Procyanidin-Rich Grape Seed Powders Enhances the Growth Performance and Muscle Crispness of Crisped Grass Carp.

Animals : an open access journal from MDPI·2026
Same author

Human-large language model collaboration in clinical medicine: a systematic review and meta-analysis.

NPJ digital medicine·2026
Same author

Corrigendum to "3D culture boosting fullerenol nanoparticles to induce calreticulin exposure on MCF-7 cells for enhanced macrophage-mediated cell removal" [Colloids and Surfaces B: Biointerfaces 224 (2023) 113204].

Colloids and surfaces. B, Biointerfaces·2025

Related Experiment Video

Updated: Apr 27, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.3K

DNA conformational flexibility study using phosphate backbone neutralization model.

Shiyan Xiao, Hong Zhu, Lei Wang

    Soft Matter
    |July 2, 2014
    PubMed
    Summary

    Metal ions significantly alter DNA structure and flexibility by changing the cation atmosphere. Phosphate neutralization affects DNA bending differently based on sequence, making GC-rich DNA less flexible and AT-rich DNA more flexible.

    More Related Videos

    Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
    08:03

    Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

    Published on: April 13, 2022

    1.8K
    Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
    09:51

    Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

    Published on: July 16, 2017

    16.2K

    Related Experiment Videos

    Last Updated: Apr 27, 2026

    Analyzing and Building Nucleic Acid Structures with 3DNA
    16:24

    Analyzing and Building Nucleic Acid Structures with 3DNA

    Published on: April 26, 2013

    20.3K
    Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
    08:03

    Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

    Published on: April 13, 2022

    1.8K
    Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
    09:51

    Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

    Published on: July 16, 2017

    16.2K

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Computational Chemistry

    Background:

    • DNA structure and physicochemical properties are crucial for cellular processes like the cell cycle.
    • The interaction between DNA and metal ions in solution influences DNA's structural and dynamic behavior.

    Purpose of the Study:

    • To investigate the effect of metal ion interplay on DNA structure and conformational flexibility.
    • To compare the behavior of a normal DNA duplex with its 'null isomer' under varying ionic conditions.

    Main Methods:

    • Utilized molecular dynamics simulations to study DNA structure and dynamic conformational behavior.
    • Analyzed the impact of phosphate neutralization on the DNA duplex and its surrounding cation atmosphere.

    Main Results:

    • Phosphate neutralization significantly alters the cation atmosphere, increasing major groove width and decreasing minor groove width.
    • Charge reduction on phosphate groups increases the probability of BI phosphate linkages and reduces global bending direction preference.
    • The effect of electrostatic changes on DNA flexibility is sequence-dependent: GC-rich sequences become less flexible, while AT-rich sequences become more flexible.

    Conclusions:

    • Metal ion interactions and phosphate neutralization profoundly impact DNA structure and dynamics.
    • Sequence-specific electrostatic effects dictate the conformational flexibility of DNA under altered ionic conditions.