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Updated: Apr 27, 2026
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
[Antimicrobial resistance in uropathogens isolated in a pediatric hospital]
Araceli Pérez-Pérez1, Leoncio Peregrino-Bejarano, Martha Camacho-Velázquez
1Departamento de Infectología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Distrito Federal México. guadalumiranda@terra.com.
Insights
Urinary tract infections are common, with Escherichia coli being the most frequent cause. High resistance to quinolones and cephalotin necessitates exploring alternative treatments for effective antimicrobial susceptibility.
Area of Science:
- Infectious Diseases
- Microbiology
- Urology
Background:
- Urinary tract infections (UTIs) are highly prevalent across all age groups.
- Increasing antimicrobial resistance poses a significant global health challenge.
- Understanding causative agents and their susceptibility is crucial for effective UTI management.
Purpose of the Study:
- To identify the primary etiologic agents responsible for UTIs.
- To determine the antimicrobial susceptibility profiles of common uropathogens.
- To inform treatment strategies in light of rising antimicrobial resistance.
Main Methods:
- A descriptive, cross-sectional survey design was employed.
- Data collected included infection type, risk factors, and antimicrobial treatments.
- Microorganism identification and antimicrobial susceptibility testing were performed.
Main Results:
- Escherichia coli was the predominant pathogen, isolated in 67% of cases.
- High resistance rates were observed for E. coli against cephalotin (58.7%) and norfloxacin (51%).
- Functional abnormalities (56%) and prophylactic antimicrobial use (76.4%) were common among patients.
Conclusions:
- Escherichia coli is the leading cause of UTIs, exhibiting significant resistance to quinolones and cephalotin.
- The high prevalence of functional abnormalities and prior antibiotic use highlights patient risk factors.
- Alternative therapeutic options beyond quinolones are urgently needed due to widespread resistance.
Background:
Urinary tract infection is one of the most common infections at all ages. Antimicrobial resistance has increased in the past few years. The aim of this study was to determine the most common etiologic agents of urinary tract infections and their antimicrobial susceptibility profiles.
Methods:
A descriptive, cross-sectional survey was conducted. Patients with a urinary tract infection identified over a 1-year period were included. The type of infection, risk condition, antimicrobial treatment, microorganism and antimicrobial susceptibility were recorded.
Statistical Analysis:
descriptive statistics.
Results:
One hundred and seventy four patients with infection, 31.4 % with urinary tract malformation, 56 % with functional abnormalities. 76.4 % were receiving antimicrobial prophylaxis. Escherichia coli was the most common agent isolated with 67%, followed by Klebsiella spp. 9 %, Pseudomonas spp. 7 % and others less frequently. Escherichia coli resistance to cephalotin was 58.7%, to norfloxacin 51 %, nitrofurantoin 15.5 %, cefuroxime 12.5 %, cefotaxime 15.5 %, cefepime 5 % and to amikacin 0 %.
Conclusions:
Escherichia coli was the most common causative agent, and resistance to quinolones and cephalotin was higher than 50 %. Most patients had urinary tract functional abnormalities and a history of prophylactic treatment use. Options other than quinolones need to be assessed due to the high resistance identified in uropathogens.
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