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Colony Forming Cell (CFC) Assay for Human Hematopoietic Cells
Published on: December 18, 2010
pp60c-src activation in human colon carcinoma
C A Cartwright1, M P Kamps, A I Meisler
1Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
The Journal of Clinical Investigation
|June 1, 1989
Summary
The protein-tyrosine kinase pp60c-src shows significantly elevated activity in human colon carcinoma cell lines and tumors. This increased activity suggests a role for activated pp60c-src in colon cancer development.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- The protein-tyrosine kinase pp60c-src is involved in cell growth and differentiation.
- Dysregulation of pp60c-src activity has been implicated in various cancers.
Purpose of the Study:
- To investigate the in vitro protein-tyrosine kinase activity of pp60c-src in human colon carcinoma.
- To determine if elevated pp60c-src activity correlates with colon cancer development.
Main Methods:
- Measurement of pp60c-src in vitro kinase activity using enolase phosphorylation and autophosphorylation assays.
- Immunoblotting with anti-phosphotyrosine antibodies to detect tyrosine-phosphorylated proteins.
Main Results:
- pp60c-src activity was significantly higher (5-8 fold) in 6/9 colon carcinoma cell lines and 13/21 primary colon carcinomas compared to normal colonic mucosa.
- Elevated pp60c-src activity was not solely due to increased protein levels, indicating enhanced specific activity.
- Colon carcinoma cells exhibited increased in vivo tyrosine phosphorylation of proteins, correlating with elevated pp60c-src kinase activity.
Conclusions:
- The specific activity of pp60c-src kinase is elevated in colon carcinoma cells and tumors.
- Activated protein-tyrosine kinase(s), likely including pp60c-src, are present in colon cancer.
- These findings suggest a potential role for pp60c-src in the pathogenesis of colon cancer.
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