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Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
Published on: November 14, 2025
603
Lysosomal membrane permeabilization in cell death: concepts and challenges.
Urška Repnik1, Maruša Hafner Česen2, Boris Turk3
1Department of Biochemistry and Molecular and Structural Biology, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia; Department of Biosciences, University of Oslo, Blindernveien 31, 0371 Oslo, Norway.
Mitochondrion
|July 2, 2014
Summary
Lysosomal membrane permeabilization (LMP) releases cellular contents, triggering cell death pathways like apoptosis or necrosis. Understanding LMP mechanisms is crucial for therapeutic applications, potentially triggering cell death or drug release.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Late endocytic compartments, including lysosomes, degrade cellular cargo.
- Lysosomal membrane permeabilization (LMP) disrupts endo/lysosomal function, releasing contents into the cytosol.
- LMP can be induced by various endogenous and synthetic agents.
Purpose of the Study:
- To explore the mechanisms and consequences of lysosomal membrane permeabilization (LMP).
- To investigate the link between LMP and various cell death pathways.
- To evaluate the therapeutic potential of targeting LMP.
Main Methods:
- Review of existing literature on LMP and cell death.
- Analysis of molecular mechanisms connecting LMP to cell death signaling.
- Discussion of methodological challenges in studying LMP.
Main Results:
- LMP can initiate apoptosis, pyroptosis, or necrosis depending on cell type and dose.
- LMP can amplify extrinsic cell death signals and inhibit autophagy.
- Mechanisms linking LMP to cell death are not fully understood, except for cathepsin involvement.
Conclusions:
- LMP is a critical event affecting endo/lysosomal function and cell fate.
- Targeting LMP offers potential therapeutic strategies for triggering cell death or controlled drug delivery.
- Further research is needed to elucidate the precise mechanisms connecting LMP and cell death signaling.
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