Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Quantum-Efficiency Blue Phosphors with Superior Thermal Stability Derived from Eu<sup>3+</sup>-Doped Faujasite Y Zeolite.

Inorganic chemistry·2023
Same author

The phosphoproteome is a first responder in tiered cellular adaptation to chemical stress followed by proteomics and transcriptomics alteration.

Chemosphere·2023
Same author

Current status and influencing factors of participating satisfaction during surgical treatment decision-making among breast cancer patients with immediate breast reconstruction.

European journal of oncology nursing : the official journal of European Oncology Nursing Society·2023
Same author

Knockdown of SIK3 in the CA1 Region can Reduce Seizure Susceptibility in Mice by Inhibiting Decreases in GABA<sub>A</sub>R α1 Expression.

Molecular neurobiology·2023
Same author

PR-957 retards rheumatoid arthritis progression and inflammation by inhibiting LMP7-mediated CD4<sup>+</sup> T cell imbalance.

International immunopharmacology·2023
Same author

ROS-mediated waterlogging memory, induced by priming, mitigates photosynthesis inhibition in tomato under waterlogging stress.

Frontiers in plant science·2023

Related Experiment Video

Updated: Apr 27, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

15.7K

Peptide-based electrochemical approach for apoptosis evaluation.

Peng Miao1, Jian Yin2, Limin Ning3

  • 1CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Biosensors & Bioelectronics
|July 2, 2014
PubMed
Summary

This study introduces a novel peptide-based electrochemical biosensor for detecting apoptotic cells. This method offers a simple, cost-effective, and sensitive approach for apoptosis evaluation.

Keywords:
ApoptosisBiosensorElectrochemistryPeptidePhosphatidylserine

More Related Videos

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.6K
Detection and Isolation of Apoptotic Bodies to High Purity
12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

10.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model
08:27

Use of a Caspase Multiplexing Assay to Determine Apoptosis in a Hypothalamic Cell Model

Published on: April 16, 2014

15.7K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.6K
Detection and Isolation of Apoptotic Bodies to High Purity
12:17

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

10.3K

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Biosensor Technology

Background:

  • Apoptosis, or programmed cell death, is crucial for development and disease.
  • Accurate detection of apoptotic cells is essential for biological studies and clinical applications.
  • Existing methods for apoptosis detection can be complex or time-consuming.

Purpose of the Study:

  • To develop a novel peptide-based electrochemical biosensor for direct apoptosis evaluation.
  • To establish a strategy for assembling apoptotic cells on a solid surface using peptides.
  • To demonstrate the biosensor's potential for sensitive and cost-effective apoptosis detection.

Main Methods:

  • Designing a peptide with a sequence to recognize externalized phosphatidylserine on apoptotic cells.
  • Immobilizing the peptide-modified electrode to capture apoptotic cells.
  • Utilizing electrochemical impedance spectroscopy to measure signal changes caused by captured cells.

Main Results:

  • The peptide successfully captured apoptotic cells onto the electrode surface.
  • Significant decrease in electrochemical signals was observed upon binding of apoptotic cells.
  • The biosensor's performance was validated against standard apoptosis detection methods.
  • The method demonstrated simplicity, cost-effectiveness, convenience, and high sensitivity.

Conclusions:

  • A novel peptide-based electrochemical biosensor for direct apoptosis evaluation has been successfully developed.
  • This biosensor provides a sensitive, cost-effective, and convenient platform for apoptosis detection.
  • The technology holds significant potential for apoptosis evaluation, therapeutic effect assessment, and cellular biology research.