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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Apr 27, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
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Cervical intraepithelial neoplasia based on array comparative genomic hybridization.

Seung Do Choi, Tae-Hee Kim, Dong Han Bae

    European Journal of Gynaecological Oncology
    |July 3, 2014
    PubMed
    Summary

    Genomic changes in cervical precancerous lesions were analyzed. Array comparative genomic hybridization identified only one upregulated gene (3q23-q24) in human papillomavirus (HPV)-positive cases compared to HPV-negative cases.

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    Area of Science:

    • Gynecologic Oncology
    • Molecular Genetics
    • Cancer Genomics

    Background:

    • Uterine cervical cancer is a common gynecologic malignancy.
    • Cervical cancer progression involves intraepithelial lesions and is linked to human papillomavirus (HPV).
    • Understanding genomic alterations in precancerous lesions is crucial for early detection and treatment.

    Purpose of the Study:

    • To identify genomic changes, specifically gene copy number alterations, in severe uterine cervical dysplasia.
    • To compare these genomic changes between HPV-positive and HPV-negative cases.
    • To assess the utility of array comparative genomic hybridization (array CGH) in evaluating cervical cancer-related genomic aberrations.

    Main Methods:

    • Array comparative genomic hybridization (array CGH) was employed to analyze gene copy number variations.
    • Five cases of severe uterine cervical dysplasia were studied: two HPV-negative and three HPV 16/18 positive.
    • Gene copy number data were compared between the HPV-positive and HPV-negative groups.

    Main Results:

    • A single gene, located at 3q23-q24, was found to be upregulated more than 1.5-fold in the HPV-positive group compared to the HPV-negative group.
    • No downregulated genes were identified between the two groups.
    • This suggests limited copy number alterations in early-stage cervical dysplasia, particularly in HPV-negative cases.

    Conclusions:

    • Array CGH is a useful tool for evaluating genomic aberrations in the context of cervical cancer development.
    • The study highlights a specific gene region (3q23-q24) with potential involvement in HPV-related cervical dysplasia.
    • Further research with larger sample sizes may reveal additional genomic alterations associated with cervical precancerous lesions.