Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amyloid Fibrils03:03

Amyloid Fibrils

10.1K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
10.1K
Amyloid Fibrils03:03

Amyloid Fibrils

5.2K
5.2K
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.2K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

5.1K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

6.1K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resistin, an adipocytokine, offers protection against acute myocardial infarction.

Journal of molecular and cellular cardiology·2007
Same author

Liquid chromatographic analysis of phosphoamino acids at femtomole level using chemical derivatization with N-hydroxysuccinimidyl fluorescein-O-acetate.

Analytica chimica acta·2007
Same author

6-oxy-(acetyl piperazine) fluorescein as a new fluorescent labeling reagent for free fatty acids in serum using high-performance liquid chromatography.

Journal of chromatography. A·2007
Same author

Synthesis and fluorescence properties of 5,7-diphenylquinoline and 2,5,7-triphenylquinoline derived from m-terphenylamine.

Molecules (Basel, Switzerland)·2007
Same author

[Metabolic engineering of terpenoids in plants].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology·2007
Same author

Hedgehog signaling in the murine melanoma microenvironment.

Angiogenesis·2007

Related Experiment Video

Updated: Apr 27, 2026

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
10:08

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

Published on: August 28, 2012

11.4K

Amyloid β regulates the expression and function of AIP1.

Huaiming Wang1, Lijing Fan1, Hong Wang1

  • 1Department of Neurology, The 89th Hospital of People's Liberation Army, 256 Beigong west Street, Weifang, 261045, Shandong Province, China.

Journal of Molecular Neuroscience : MN
|July 3, 2014
PubMed
Summary

Apoptosis signal-regulating kinase 1-interacting protein-1 (AIP1) promotes Alzheimer's disease (AD) pathology by mediating amyloid-beta (Aβ) induced apoptosis. AIP1 facilitates Aβ-induced ASK1 activation, suggesting a novel therapeutic target for AD.

More Related Videos

Aip1p Dynamics Are Altered by the R256H Mutation in Actin
08:57

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Published on: July 30, 2014

7.8K
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
09:52

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Published on: December 28, 2017

10.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain
10:08

Purification and Aggregation of the Amyloid Precursor Protein Intracellular Domain

Published on: August 28, 2012

11.4K
Aip1p Dynamics Are Altered by the R256H Mutation in Actin
08:57

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Published on: July 30, 2014

7.8K
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
09:52

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Published on: December 28, 2017

10.3K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Alzheimer's disease (AD) involves complex cellular mechanisms.
  • Apoptosis signal-regulating kinase 1-interacting protein-1 (AIP1) is a Ras GTPase-activating protein family member linked to apoptosis.
  • The role of AIP1 in AD pathogenesis remains unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of AIP1 in Alzheimer's disease (AD).
  • To determine the effect of amyloid-beta (Aβ) on AIP1 expression and activity in brain cells.

Main Methods:

  • Western blotting and co-immunoprecipitation assays were used to analyze protein interactions and levels.
  • Aβ1-42 treatment was applied to Tg2576 mouse brains and cerebral microvascular endothelial cells (CECs).
  • RNA interference (siRNA) was employed to silence AIP1 expression.

Main Results:

  • AIP1 levels were elevated in AD mouse brains and Aβ1-42 treated CECs.
  • Aβ1-42 induced AIP1 interaction with ASK1, causing dissociation from 14-3-3 and ASK1 activation (Ser-967 dephosphorylation).
  • AIP1 silencing prevented Aβ-induced ASK1 activation, JNK/p53 signaling, and CEC apoptosis.

Conclusions:

  • AIP1 mediates Aβ-induced ASK1 activation by disrupting the ASK1-14-3-3 complex.
  • AIP1 plays a critical role in Aβ-induced apoptosis in cerebral microvascular endothelial cells.
  • AIP1 represents a potential therapeutic target for mitigating apoptosis in Alzheimer's disease.