Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.1K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.1K
Two-Dimensional Force System01:20

Two-Dimensional Force System

1.9K
A two-dimensional system in mechanical engineering involves the analysis of motion and forces in a plane. A two-dimensional force vector can be resolved into its components as:
1.9K
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

3.2K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
3.2K
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

1.3K
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
1.3K
Three-Dimensional Force System01:30

Three-Dimensional Force System

3.2K
In mechanical engineering, a three-dimensional force system is a system of forces acting in three dimensions, with forces applied along the x, y, and z coordinate axes. The three-dimensional force system is an important concept in mechanical engineering, as it allows engineers to understand and analyze the behavior of objects and structures in three dimensions. By understanding the forces acting on a system, engineers can design more efficient and effective mechanical systems that can withstand...
3.2K
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

2.1K
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phototropin2 LOV1 plays a role in strong light signal transduction leading to chloroplast avoidance response.

Acta physiologiae plantarum·2026
Same author

Sensitivity of Chloroplast Movements Along an Elevational Gradient Among Three Alpine Plant Species With Different Life History Strategies.

Physiologia plantarum·2026
Same author

Reversible phosphorylation of NPH3/RPT2-like proteins regulates phototropin receptor signaling.

The Plant cell·2026
Same author

Chloroplast Movement Imaging Under Different Light Regimes With a Hyperspectral Camera.

Bio-protocol·2025
Same author

GLR Channels Are Involved in the Mechanism of Chloroplast Avoidance Response in <i>Lemna trisulca</i>.

Plants (Basel, Switzerland)·2025
Same author

Effect of UV-A on endophyte colonisation of Arabidopsis thaliana.

PloS one·2025

Related Experiment Video

Updated: Apr 27, 2026

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

3.5K

AtomicJ: an open source software for analysis of force curves.

Paweł Hermanowicz1, Michał Sarna2, Kvetoslava Burda2

  • 1Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.

The Review of Scientific Instruments
|July 3, 2014
PubMed
Summary

AtomicJ is an open-source Java application for analyzing Atomic Force Microscope data. It rapidly generates maps of mechanical properties and reveals how sample deformation changes with indentation depth.

More Related Videos

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
10:10

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

Published on: July 20, 2022

5.7K
Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
13:04

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Published on: January 18, 2022

4.5K

Related Experiment Videos

Last Updated: Apr 27, 2026

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid
08:58

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

3.5K
Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy
10:10

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

Published on: July 20, 2022

5.7K
Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
13:04

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Published on: January 18, 2022

4.5K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biophysics

Background:

  • Atomic Force Microscopy (AFM) generates complex force curve and image data.
  • Analysis of AFM data requires specialized software for extracting mechanical properties.

Purpose of the Study:

  • To introduce AtomicJ, an open-source Java application for AFM data analysis.
  • To provide a versatile tool for analyzing force curves and images.

Main Methods:

  • AtomicJ supports various contact mechanics models.
  • It implements procedures to minimize deviations from contact models.
  • Concurrent multi-threading enables high-speed analysis.

Main Results:

  • Generates maps of mechanical properties (Young's modulus, adhesion force, sample height).
  • Calculates indentation depth-dependent deformation response (stacks).

Conclusions:

  • AtomicJ offers efficient and comprehensive analysis of AFM data.
  • The application is cross-platform compatible (Windows, Linux, Macintosh).