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Related Experiment Video

Updated: Apr 27, 2026

Measurement of Cellular Chemotaxis with ECIS/Taxis

Measurement of Cellular Chemotaxis with ECIS/Taxis

Published on: April 1, 2012

Eosinophil chemotaxis.

¹Institute of Science & Technology in Medicine, Keele University, Keele, Staffordshire, ST5 5BG, UK, g.dent@keele.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|July 3, 2014

Summary

This summary is machine-generated.

This study presents a new 96-well assay for quantifying eosinophil migration, enabling automated measurements for immune and inflammatory studies. This method efficiently uses blood samples for robust chemotaxis analysis.

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Area of Science:

Immunology
Cell Biology
Biomedical Assays

Background:

Chemotaxis assays are crucial for studying leukocyte behavior in immune and inflammatory conditions.
Multiwell assays facilitate high-throughput measurements.
Fluorescence-based methods and microplate chambers enable assay automation.

Purpose of the Study:

To describe a quantitative method for measuring eosinophil migration.
To adapt chemotaxis assays for a 96-well format.
To enable efficient cell analysis from blood samples.

Main Methods:

Development of a 96-well microplate-based chemotaxis chamber.
Utilizing fluorescence detection for quantitative measurement.
Employing cell numbers obtainable from typical blood samples.

Main Results:

Successful quantitative measurement of eosinophil migration.
Demonstration of a high-throughput, automated assay.
Validation of the method with realistic blood cell yields.

Conclusions:

The described 96-well assay provides an efficient and automatable method for studying eosinophil chemotaxis.
This technique is valuable for research in leukocyte biology and immune/inflammatory pathology.
The assay is practical for laboratories working with limited cell numbers from blood samples.