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Author Spotlight: Investigating the Pathophysiology of Eosinophilic Esophagitis
Published on: May 10, 2024
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Interactions of eosinophils with nerves
Quinn R Roth-Carter1, David B Jacoby, Zhenying Nie
1Pulmonary and Critical Care Medicine, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, L334, 97239-3098, Portland, OR, USA, rothcart@ohsu.edu.
Methods in Molecular Biology (Clifton, N.J.)
|July 3, 2014
Summary
Researchers developed methods to coculture human eosinophils with parasympathetic ganglia and dorsal root ganglia. This technique allows for studying interactions between these crucial cell types in vitro.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Eosinophils and nerve cells interact in various physiological and pathological processes.
- Understanding these interactions requires specialized in vitro models.
Purpose of the Study:
- To describe established methods for coculturing human eosinophils with primary neuronal tissues.
- To provide a reproducible technique for studying neuro-immune cell interactions.
Main Methods:
- Isolation and culture of human parasympathetic ganglia.
- Isolation and culture of human dorsal root ganglia.
- Integration of primary eosinophils into established neuronal cultures.
Main Results:
- Successful establishment of cocultures containing human eosinophils and primary neurons.
- Demonstration of a viable method for studying neuro-eosinophil interactions in vitro.
Conclusions:
- The described methods enable the study of direct interactions between human eosinophils and primary neurons.
- This coculture system is a valuable tool for investigating neuro-immune mechanisms in health and disease.
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